临床神经外科杂志Issue(2)：90-93,4.DOI:10.3969/j.issn.1672-7770.2014.02.003
miR-125 b对胶质瘤细胞侵袭能力影响的体内研究
Research on miR-125 b expression and invasion of glioma in vivo
摘要
Abstract
Objective To investigate the function and possible mechanisms of miR-125 b expression on the invasion of glioma in vivo .Methods Primary glioblastoma cells were separated and cultured , which were transfected with lentiviral vectors for miR-21 mimics or miR-21 inhibitors for upregulation or downregulation of miR-125b expression levels.After that, cells were directly injected subcutaneously into the flanks of nude mice .The invasive effects of primary glioblastoma cells and stem cells were measured by Transwell assay , invasion related genes were screened by mRNA array, and the expressing levels of matrix metalloprotease ( MMP)-2, MMP-9, RECK and TIMP3 were identified by Western blot .Results miR-125 b mimics transfection in vitro could effectively improve the expression of miR-125 b in primary glioblastoma cells and stem cells , miR-125 b inhibitor transfection could effectively inhibit the expression of miR-125 b.Results showed that miR-125 b mimics or inhibitor had no effects on the invasion of primary glioblastoma cells in vivo , and did not affect the expression of MMP-2, MMP-9, RECK and TIMP3.Conclusion miR-125b expression is not related to the invasion properties of primary glioblastoma cells in vivo .关键词
胶质瘤/miR-125b/侵袭Key words
glioma/miR-125 b/invasion分类
医药卫生引用本文复制引用
李晓良,孙王伟,张曙光,万意,石磊,王之敏,王存祖..miR-125 b对胶质瘤细胞侵袭能力影响的体内研究[J].临床神经外科杂志,2014,(2):90-93,4.基金项目
国家自然科学基金项目资助（81000963），江苏省“333工程”培养资金资助项目（BRA2011046）；江苏省卫生厅医学科研课题（ YG201301）；苏州社会发展项目基金（ SYS201063）；昆山社会发展项目基金（ KS1006和 KS1009）；江苏大学医学临床科技发展基金项目 （）
