中国临床保健杂志Issue(2)：157-159,3.DOI:10.3969/J.issn.1672-6790.2014.02.017
不同浓度粒巨细胞集落刺激因子及白介素-4对树突状细胞体外诱导培养的影响
Generation of dendritic cells with appropriate concentration of granuIocyte-macrophage coIony-stimuIating factor and interleukin-4
摘要
Abstract
Objective To investigate the appropriate concentration range of murine granulocyte-macrophage colony-stimulating factor(GM-CSF)and interleukin-4(IL-4)in incubating mouse bone marrow monocytes-like cells and proper conditions for dendritic cell differentiation.Methods The progenitor of dendritic cells were isolated from bone marrow of Balb/c mice,and incubated in different dose of mouse granuIocyte-macrophage coIony-stimuIating fac-tor and interleukin-4 for continuously seven days,the morphology of cells in each group were observed under micro-scope and snapped with digital camera every other day.The expression of CD1 1 c was detected with flow cytometry. Results Morphological observation revealed that mouse bone marrow monocytes-like cells changed remarkably when cultured with low dose of cytokine.Cells became large and showed dendritic protruding on their surface.Furthermore, most cells were CD1 1 c positive when detected by flow cytometry.The appropriate GM-CSF and IL-4 dose range were 5μg/L~100 μg/L and 2.5 μg/L~50 μg/L respectively,while cytokines of overhigh concentration cause cell death. Conclusion Dendritic cells can be derived from bone marrow monocytes-like cells with appropriate dose of GM-CSF and IL-4 treatment with High purity DCs.关键词
树突细胞/细胞培养技术/巨噬细胞集落刺激因子/白细胞介素4Key words
Dendritic cells/Cell culture techniques/Macrophage colony-stimulating factor/Interleukin-4分类
医药卫生引用本文复制引用
王晓婧,梅晓冬..不同浓度粒巨细胞集落刺激因子及白介素-4对树突状细胞体外诱导培养的影响[J].中国临床保健杂志,2014,(2):157-159,3.基金项目
2013年度安徽省自然科学基金项目 （）
