中国临床保健杂志Issue(2)：160-162,3.DOI:10.3969/J.issn.1672-6790.2014.02.018
吡格列酮对高糖培养大鼠肾小球系膜细胞p38丝裂原活化蛋白激酶和转化生长因子β-1的影响
Effects of pioglitazone on the expressions of p38 mitogen-activated protein kinase and transforming growth factor β-1 in cultured rat glomerular mesangial cells
摘要
Abstract
Objective To observe the effects of pioglitazone on the expressions of p38 mitogen-activated pro-tein kinase (p38MAPK)and transforming growth factor β-1 (TGF-β1 )in cultured rat glomerular mesangial cells (MCs)and explore its reno-protective mechanism.Methods MCs were cultured in the medium with normal glucose concentration (group NG),high glucose concentration (group HG),p38MAPK special inhibitor(group S)and piogli-tazone (group P).The expression levels of phosphory1ated p38MAPK (p-p38)and total p38MAPK (t-p38)were measured by Western blot.The expressions of TGF-β1 mRNA were detected by semiquantitative RT-PCR.Results Compared with group NG,the p38MAPK activity and TGF-β1 mRNA expression increased significantly (P<0.01)in group HG.Compared with group HG,p38MAPK special inhibitor inhibited markly HG-induced p38MAPK activity and TGF-β1 expression (P<0.05).When treated with pioglitazone,p38MAPK activity and TGF-β1 expression decreased (P<0.05),which were similar to group S.p38MAPK activity was positively correlated with TGF-β1 expression (r=0.587,P<0.01).Conlusions Pioglitazone can suppress TGF-β1 expression of MCs via p38MAPK pathway,which may contribute partly to its reno-protection.关键词
糖尿病肾病/肾小球系膜细胞/P38丝裂原活化蛋白激酶类/转化生长因子β1/噻唑烷二酮类Key words
Diabetic nephropathies/Mesangial cells/p38 mitogen-activated protein kinases/Transforming growth factor beta 1/Thiazolidinediones分类
医药卫生引用本文复制引用
汪姗,叶山东,孙文佳,胡圆圆..吡格列酮对高糖培养大鼠肾小球系膜细胞p38丝裂原活化蛋白激酶和转化生长因子β-1的影响[J].中国临床保健杂志,2014,(2):160-162,3.基金项目
安徽省自然科学基金（11040606M161）；安徽高校省级自然科学研究项目 （）
