岭南现代临床外科Issue(2)：122-126,5.DOI:10.3969/j.issn.1009-976X.2014.02.003
MALAT1对膀胱癌UMUC3细胞功能的影响及下游基因的筛选
The impact of MALAT1 on cellular function of bladder cancer cell line UMUC3 and its downstream target genes
摘要
Abstract
Objective To investigatethe effect of MALAT1 on regulationof proliferation, migration, invasion , clone formation , and other functions in UMUC3 cell lines of bladder cancer , and to explore the molecular mechanism of MALAT1 in bladder cancer. Methods Thesmall interfering RNA (siRNA) was designed and synthesized and then transfected into the UMUC3 cells. The transfection efficiency was detected with RT-QPCR assay. MTS assay , transwell migration and matrigel invasion assay , colony formation assay and 5-ethinyl-2′-deoxyuridine (EDU) assay were performed to evaluate the proliferation, migration, invasion, clone formation and cell cycle respectively. To explore the potential pathways of MALAT1, we performed gene chip assay. Results After down regulation the expression of MALAT1 , the proliferation , invasion , migration and colony-forming ability of UMUC3 bladder cancer cells were impaired. Gene chip found that cancer pathways related gene changed obviously after knockdown of MALAT1 in UMUC3 cells. Conclusion MALAT1 promoted the proliferation, invasion, migration , cell cycle and clone formation ability in UMUC3 cell lines of bladder cancer through modulating the cancer pathways related gene activation and deactivation.关键词
膀胱肿瘤/MALAT1/RNA 干扰/基因芯片/癌症通路Key words
Bladder cancer/MALAT1/RNA interference/Gene chip/Pathway in cancer分类
医药卫生引用本文复制引用
王沛,江春,何旺,林天歆,黄健..MALAT1对膀胱癌UMUC3细胞功能的影响及下游基因的筛选[J].岭南现代临床外科,2014,(2):122-126,5.基金项目
国家自然科学基金 （）
