摘要
Abstract
Objective:To screen herbs with sunscreening and depigmenting effect from a large number of natural plants or herbs for providing new therapy of skin hyperpigmentation disorders. Methods: Firstly, ancient Chinese medical and contemporary literatures were reviewed to pick out herbs which may have depigmenting potential. Then, these herbs were screened by UV spectrophotometry 200 nm~400 nm wavelength scaning to determine UVA and UVB absorption, mushroom tyrosinase inhibitory activities assay to detect to detect the absorption value (A490) by a microplate reader at 490 nm wavelength,and DPPH radical scavenging method to detect DPPH clearance and DPPH half clearance (IC50). Results:From ancient and modern literatures, 15 herbs were picked out.Among these herbs, the UVA absorption values of flos sophorae, aloes and angelica dahurica benth increased significantly compared with those of control group (P<0.01), while the UVB ones of scutellaria baicalensis georgi, flos sophorae and aloes remarkably excelled in those of control group (P < 0.05). Meanwhile, the tyrosinase inhibition rate of aloes, atractylodes macrocephala koidz, flos sophorae, tuckahoe, rhizoma bletillae striatae and ginseng ascent moderately, especially that of aloes and atractylodes macrocephala koidz greatly outweighed the control group (P<0.05). In addition, the above-mentioned herbs played roles of DPPH scavenging on various degrees, among which the DPPH clearance and IC50 of flos sophorae and scutellaria baicalensis georgi were close to those of control group (P> 0.05). Eventually, 10 herbs were selected:ginseng, tuckahoe, atractylodes macrocephala koidz, semen coicis, platycodon grandiflorum, radix astragali mongolici, scutellaria baicalensis georgi, flos sophorae, aloes and rhizoma bletillae striatae. Conclusions: These 10 herbs have broad-spectrum UV absorption, tyrosinase activity inhibiton, and anti-free radical oxidation, which may lay an important foundation for further experiments of treating hyperpigmentation dermatoses.关键词
防晒/祛色素/色素增加性皮肤病/天然中草药/筛选Key words
Sunscreen/Depigmentation/Skin hyperpigmentation disorders/Natural herb/Screening分类
临床医学
