广东石油化工学院学报Issue(6):24-26,3.
酶法提取罗非鱼鱼鳍胶原蛋白的研究
On the Extraction of Collagen Protein from Tilapia Fin with Papain
王春 1周天 1方素云1
作者信息
- 1. 广东石油化工学院化学与生命科学学院,广东茂名525000
- 折叠
摘要
Abstract
The extraction of collagen protein from tilapia fin is studied .The single factor experiment was conducted to study the effects of dif-ferent factors on pepsin hydrolysis of tilapia ,and the best hydrolysis technology of tilapia pepsin hydrolysis were determined by orthogonal test .The results showed that the better effect of pepsin hydrolysis is in the enzymolysis time 6h ,substrate concentration 5% ,the amount of enzyme 5500 U/g ,pH 1 .80 ,the temperature 40 ℃ .Orthogonal test showed that the order of effect is :reaction time> enzyme amount>substrate concentration .关键词
罗非鱼鱼鳍/胃蛋白酶/胶原蛋白Key words
tilapia fin/pepsin hydrolysis/collagen protein分类
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王春,周天,方素云..酶法提取罗非鱼鱼鳍胶原蛋白的研究[J].广东石油化工学院学报,2013,(6):24-26,3.