国际眼科杂志Issue(3)：399-401,3.DOI:10.3980/j.issn.1672-5123.2014.03.05
膳食诱导的肥胖型 C57 BL／6小鼠视网膜神经节细胞凋亡的机制
Mechanism of retinal ganglion cells apoptosis in the diet -induced obese C57 BL/6 mice
摘要
Abstract
AIM:To investigate the mechanism of retinal ganglion cells (RGCs) apoptosis in the diet-induced obese C57BL/6 mice. <br> METHODS: Mice were fed high-fat diet. After 19 weeks of feeding, the mice were divided into diet induced obesity-resistant ( DIO-R ) group and diet induced obesity ( DIO) group, while mice of the control ( CON) group were fed a basal diet at the same time. The apoptosis of RGCs was detected by TUNEL.Laser scanning confocal microscope was used to detect the intracellular calcium ion concentration. <br> RESULTS: TUNEL staining showed apoptosis cells in ganglion cell layer ( GCL) in DIO group increased and the percentage of apoptotic cells was (6.7±1.2)%which was much higher than in CON and DIO-R groups ( P<0.01, P<0.05).There was no significant difference between CON group and DIO-R group ( P>0.05 ).Laser scanning confocal microscope detection showed Ca2+ staining intensity of RGCs in DIO group increased and its staining intensity was significantly higher than in CON and DIO-R mice (P<0.01,P<0.01), whereas there was no significant difference between CON group and DIO-R group ( P>0.05 ) . <br> CONCLUSION:Intracellular calcium ion overload might be involved in the RGCs apoptosis in the diet-induced obese C57BL/6 mice.关键词
肥胖/视网膜神经节细胞/凋亡/钙离子浓度Key words
obesity/retinal ganglion cells/apoptosis/calcium ion concentration引用本文复制引用
白霞,赵剑,赵文青,陈玉玲..膳食诱导的肥胖型 C57 BL／6小鼠视网膜神经节细胞凋亡的机制[J].国际眼科杂志,2014,(3):399-401,3.基金项目
国家自然科学基金资助项目（ No．81202227）；辽宁省科技厅博士启动基金项目（ No．20121122）@@@@National Natural Science Foundation of China （ No．81202227）； Doctoral Initiating Fund of Science and Technology Department of Liaoning Province ， China （）
