国际眼科杂志Issue(4)：610-613,4.DOI:10.3980/j.issn.1672-5123.2014.04.09
糖尿病血糖波动动物模型建立的初探
Discussion on the establishment of blood glucose fluctuation animal models
盖春柳 1赵静如 2陈晓隆1
作者信息
- 1. 110004 中国辽宁省沈阳市，中国医科大学附属盛京医院眼科
- 2. 100700 中国北京市，北京中医药大学东直门医院
- 折叠
摘要
Abstract
AIM:To provide the experimental basis for the in vivo study of blood glucose fluctuation injury mechanism, through intraperitoneal injection of glucose to establish blood glucose fluctuation animal models and to simulate blood glucose fluctuation of patients with diabetes. <br> METHODS: Rats were randomly divided into four groups:normal control group (NC), normal fluctuation group ( NF) , diabetes mellitus group ( DM) and diabetes fluctuation group ( DF ) .Diabetic models were induced through intraperitoneal injection of STZ. A certain amount of glucose was injected in the rats of group NF and DF intraperitoneally three times a day after the model was established, thereby causing blood glucose fluctuations.Full-day blood glucose monitoring was conducted once every two weeks to observe the blood glucose fluctuation situation, according to blood glucose levels drawing the “time-blood glucose concentration”curve.The stability of blood glucose was evaluated from four different aspects, through calculating mean blood glucose ( MBG ) , standard deviation blood glucose ( SDBG ) , largest amplitude of glycemic excursions ( LAGE) and Sclichtkrull Mz value. <br> RESULTS: The full-day blood glucose of rats in the group NC was in a normal range, and it was stable without fluctuation.The blood glucose of rats in group NF was fluctuated between 5-10mmol/L. The blood glucose of rats in group DM were maintained at higher level (>20mmol/L), without great fluctuation extent and DF with sighificant fluctuation. The blood glucose fluctuation in graoup DF was significant. The blood glucose changes of rats in the group NF and DF were significant and regular. The curve of blood glucose fluctuations was relatively stable.All values of rats in group NF, DM and DF were significantly increased compared with group NC.Group DF was increased more significantly, and the comparison with group NF and DM had significant difference (P<0.01). <br> CONCLUSION: Significant blood glucose fluctuation can be formed through the method of manual intraperitoneal injection of glucose.The method can be used for simulating the blood glucose fluctuation situation of patients with diabetics.This method has the advantages of simple operation, low injury and good repeatability.It can provide experimental basis for in vivo study of blood glucose fluctuation injury mechanism and pathogenesis of diabetic complications.关键词
糖尿病/血糖波动/动物模型Key words
diabetes mellitus/blood glucose fluctuation/animal model引用本文复制引用
盖春柳,赵静如,陈晓隆..糖尿病血糖波动动物模型建立的初探[J].国际眼科杂志,2014,(4):610-613,4.
