国际眼科杂志Issue(5)：815-818,4.DOI:10.3980/j.issn.1672-5123.2014.05.07
VEGF和MMP-9在大鼠视网膜缺血再灌注损伤中表达及相关性研究
Expression levels and correlation of VEGF and MMP - 9 in rat retinal ischemia/reperfusion injury
吴鹏程 1张文芳 1张书1
作者信息
- 1. 730030 中国甘肃省兰州市，兰州大学第二医院眼科
- 折叠
摘要
Abstract
AIM:To investigate the expression levels and correlation of VEGF and MMP-9 in the retinal ischemia/reperfusion injury( RIRI) . <br> METHODS: Eighty-four Wistar rats were divided into normal control group and at 6h, 12h, 24h, 48h, 3d and 7d group after retinal ischemia/reperfusion. The model of RIRI was constructed by elevating the intraocular pressure. The expression levels of VEGF and MMP-9 was measured using immunohistochemical staining. <br> RESULTS:No VEGF positive cells were found in normal group and it began to express at 12h, increased gradually and reached maximal levels at 48h after RIRI. MMP-9 positive expression was rarely detected in the normal control group and began to increase at 6h, reached the peak at 24h. There was statistical difference between normal control group and ischemia/reperfusion group ( P<0. 01) with regard to the expression of VEGF and MMP-9. The expression of VEGF was positively correlated to MMP-9(P<0. 05). <br> CONCLUSION:The high expression of VEGF and MMP-9 is significantly correlated with RIRI and may play an important role in the occurrence and development of retinal ischemic injury.关键词
视网膜/缺血再灌注损伤/血管内皮生长因子/基质金属蛋白酶Key words
retina/ischemia/reperfusion injury/vascular endothelial growth factor/matrix metalloprotelnase-9引用本文复制引用
吴鹏程,张文芳,张书..VEGF和MMP-9在大鼠视网膜缺血再灌注损伤中表达及相关性研究[J].国际眼科杂志,2014,(5):815-818,4.
