中南医学科学杂志Issue(2)：139-143,5.
脂多糖对小鼠巨噬细胞ABCA1表达和胆固醇流出的影响
The Effect of Lipopolysaccharide-Induced Acute Phase Reaction on Expression of ABCA1 in Macrophages of Mice
摘要
Abstract
Objective To investigate the effect of lipopolysaccharide(LPS) on the expression of the ABCA1 and cholesterol efflux in macrophages of mice,the role of LXRα in this process was also observed. Methods 60 C57 mice were divided into two groups. The experimental group were treated with LPS (1. 0 mg/kg). The control group were treated with PBS (1. 0 mg/kg). After 12 h,the mRNA and protein expression of ABCA1 and LXRα in peritoneal macrophages of mice were examined by reverse transcriptase-polymerase chain reaction( RT-PCR) and Western-blot. The cholesterol efflux of macrophages was determined by liquid scintillator. The cells were treated with LXRαagonist 22(R)-Hch,and the role of LXRα in the regulated effect of LPS on the expression of ABCA1 were observed. Results LPS decreased the expression of LXRα and ABCA1 in peritoneal macrophages of mice. And the cholesterol efflux of macrophages was also inhibited by LPS. After treated with 22(R)-Hch,the inhibited effect of LPS on expression of ABCA1 and cholesterol efflux were obvious-ly abolished in mice macrophages. LPS-induced acute phase reaction down-regulated the expression of ABCA1 and de-creased cellular cholesterol efflux in macrophages of mice,which may be related to the LXRα-dependent pathway. Con-clusion Inhibiting the activation of signaling pathways TLR4/LXRα,which can reduce inflammation during LPS-induced APR response and increase expression of ABCA1 to promote the outflow of intracellular cholesterol.关键词
脂多糖/肝X受体α/炎症/三磷酸腺苷结合盒转运体A1Key words
lipopolysaccharide/liver X receptor α/acute phase reaction/ATP-binding cassette transporter A1分类
医药卫生引用本文复制引用
曾志青,李金凤,谢笛,汤石林..脂多糖对小鼠巨噬细胞ABCA1表达和胆固醇流出的影响[J].中南医学科学杂志,2014,(2):139-143,5.基金项目
衡阳市科技计划项目(2010kj17,2010kj41),国家级大学生创新创业训练计划(201210555018),湖南省研究生科研创新项目(2012SCX13) （2010kj17,2010kj41）
