南京林业大学学报（自然科学版）Issue(1)：1-8,8.DOI:10.3969/j.issn.1000-2006.2014.01.001
杉木地理种源的EST-SSR分子标记变异研究
Variation of EST-SSR molecular markers among provenances of Chinese fir
摘要
Abstract
In order to detect the genetic variation of Chinese fir provenance, 42 Chinese fir provenance samples were se-lected for genetic diversity analysis based on EST-SSR makers. Experimental results showed that there was higher genetic polymorphism among Chinese fir provenance. Base on the 17 locus amplified by the 15 EST-SSR pair primers, a total of 52 polymorphic alleles with average of 3.058 8 alleles for per locus were found. The average PIC was 0.296 for each lo-cus. Comparing to others makers, more genetic variation information could be detected by SSR. Except provenance No. 2 and provenance No. 16, the other 40 provenances could be classified into four groups, with genetic distance of 10 as threshold. Our results fit well with the formal research on Chinese fir seed zones map based on growth and adaptive traits. In this study, we also found that there were some SSR variations located in the coding area of functional genes responding to certain biological or non-biological stress. The authors suggested that the geographic variation of Chinese fir might be due to these allelic variations of genes or in other words, the adaptive variation and local fixation.关键词
杉木种源/SSR/地理等位变异/进化Key words
Chinese fir provenance/SSR/geographical allelic variation/evolution分类
农业科技引用本文复制引用
徐阳,张志才,黄金华,叶代全,方扬辉,陈金慧,赵亚琦,王颖,王新民,刘伟东,施季森,郑仁华,欧阳磊..杉木地理种源的EST-SSR分子标记变异研究[J].南京林业大学学报（自然科学版）,2014,(1):1-8,8.基金项目
国家林业公益性行业科研专项项目（201004049）；国家自然科学基金重点项目（30930077）；江苏高校优势学科建设工程资助项目 （）
