南京林业大学学报（自然科学版）Issue(1)：21-26,6.DOI:10.3969/j.issn.1000-2006.2014.01.004
杉木育种群体SSR分子标记遗传多样性分析
Genetic diversity among the germplasm collections of the Chinese fir in 1st breeding population upon SSR markers
摘要
Abstract
It is the core strategy of multi-generation genetic improvement to maintain a reasonable genetic diversity and clear genetic background in breeding populations during the Chinese fir genetic improvement. The genetic materials, which were conserved in the Chinese national germplasm bank, were analyzed systematically by 52 pairs of SSR markers developed. The genetic diversity in the 1st breeding populations was evaluated based upon the molecular level of SSR markers. The results showed that there were 254 polymorphic SSR loci detected by 52 pairs of SSR primers, of which each SSR marker produced polymorphic loci alleles ranged from 2 to 8, and all sites observed number of alleles, num-bers of effect alleles, observed heterozygosity, Nei�s gene diversity and Shannon information index were 4. 88, 2�32, 0�43, 0�50 and 0. 98, respectively. The five evaluation indices for the level of genetic diversity were consistent. Ob-served number of alleles, effectives numbers of alleles, observed heterozygosity, Nei�s gene diversity and Shannon infor- mation index were different among 52 pairs of SSR markers, and their coefficient of variation were 24.88%,44�75%, 34�20%,34�89% and 33�59%, respectively. The average value of genetic identities was 0. 693 3 among 93 samples, ranged from 0�490 0 to 0�919 3;the average value of genetic distance was 0.370 3, ranged from 0.086 3 to 0.713 4. And according to the UPGMA cluster analysis, the 93 samples could be obviously divided into five main groups based on genetic distance ( D=20) , When the genetic distance value was set at 15( D=15) , the fifth group ( including 77 germ-plasm) could be divided into 16 sub-groups. UPGMA clustering results indicated that most of the collections could be grouped upon their original geographic sites to be selected;even those relationships between germplasm classification and geographic origin would be very complicated.关键词
杉木/育种群体/SSR标记/遗传多样性管理Key words
Cunninghamia lanceolata/breeding populations/SSR markers/genetic diversity management分类
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欧阳磊,陈金慧,郑仁华,徐阳,林宇峰,黄金华,叶代全,方扬辉,施季森..杉木育种群体SSR分子标记遗传多样性分析[J].南京林业大学学报（自然科学版）,2014,(1):21-26,6.基金项目
国家林业公益性行业科研专项项目（201004049）；福建省林木种苗科技攻关三期项目（闽林科［2009］4号）；福建省省属公益类科研院所基本科研专项项目 （）
