南京林业大学学报（自然科学版）Issue(1)：151-156,6.DOI:10.3969/j.issn.1000-2006.2014.01.027
基于DNA的木材树种识别研究进展
Progress of wood identification based on DNA methods
摘要
Abstract
From wood identification points of view, DNA extraction methods ( CTAB, SDS, PTB, DNeasy Plant Mini Kit etc.) and DNA barcode and fingerprint and wood identification methods based on DNA were summarized. For the DNA barcode concerned, the emphases were on the signature sequences in genome DNA ( chloroplast DNA, cpDNAr-bcL,matK,trnH-psbAintergenic spacer sequences,and ribosomal DNA, rDNA ITS) as well as identification form ( varia-ble sites and phylogenetic tree) described. For the DNA fingerprint method,the emphases were on the progress and out-look of 4 kinds of molecular makers such as random amplificated polymorphism DNA, RAPD,inter-simple sequence re-peat, ISSR、simple sequence repeats, SSR,single nucleotide polymorphism, SNP. It was concluded that wood identifica-tion based on DNA methods is feasible in theory but needs more studied on.关键词
木材树种识别/DNA提取/DNA条形码/DNA指纹图谱Key words
wood identification/DNA extraction/DNA barcode/DNA fingerprint分类
农业科技引用本文复制引用
张蓉,殷亚方,徐魁梧,叶克林..基于DNA的木材树种识别研究进展[J].南京林业大学学报（自然科学版）,2014,(1):151-156,6.基金项目
国家林业公益性行业科研专项项目（201304508）；江苏高校优势学科建设工程资助项目 （）
