南京林业大学学报（自然科学版）Issue(1)：183-185,3.DOI:10.3969/j.issn.1000-2006.2014.01.033
枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定
Rapid identification of Carpomya vesuviana Costa and five relative species of fruit flies by PCR-RFLP
摘要
Abstract
Carpomya vesuviana Costa has been a new quarantine pest in China. In this paper, the method of rapid identi-fication of C. vesuviana Costa and 5 relative species of fruit flies was studied based on PCR-RFLP of the mitochondrial DNA( mtDNA) . The results showed that DNAs, which were estimated about 350 bp and 450 bp respectively, were ob-tained by means of PCR with two pairs of primers designed from mtDNA of target pests.The PCR-products could be reg-istered by restriction enzymes ( Dra I and Mse I) . All the 6 species could be discriminated according to the banding pat-terns of the restriction enzymes. It is suggested that the method is reliable for quick discrimination of fruit flies.关键词
枣实蝇/检疫性实蝇/PCR-RFLP/线粒体DNAKey words
Carpomya vesuviana Costa/quarantine fruit flies/PCR-RFLP/mtDNA分类
生物科学引用本文复制引用
程晓甜,阿地力·沙塔尔,张伟,李新泉..枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定[J].南京林业大学学报（自然科学版）,2014,(1):183-185,3.基金项目
新疆维吾尔自治区科技计划项目（201130102-3）；新疆维吾尔自治区重点学科森林培育科研项目 （）
