南京林业大学学报（自然科学版）Issue(2)：52-56,5.DOI:10.3969/j.issn.1000-2006.2014.02.011
青钱柳愈伤组织不定芽诱导技术
Induction techniques of adventitious shoot from callus of Cyclocarya paliurus in vitro
摘要
Abstract
Based on the seedlings cultured with matured seeds of Cyclocarya paliurus in vitro, the induction of adventi-tious shoot from callus of C. paliurus and the effects of basic media and different growth regulators on induction of callus and adventitious shoots were studied with explants of leaves and stem segments of the young seedlings. The results showed that callus of leaves and stem segments of the seedlings could be induced on each basic medium applied in this paper and the highest induction frequency of callus reached 90.00% for stem segment and 86.70% for leaf, respectively. The types and concentrations of hormones had significant effects on callus induction. When 0.5 mg/L TDZ was added, the induction frequency of leaf callus reached 95.00%, while 92.85% induction frequency was achieved for stem segment callus at 0.5 mg/L TDZ added. The greatest induction frequency of adventitious shoots from leaf callus was observed in the medium of WPM+1.0 mg/L 6-BA+0.1 mg/L IBA, which reached to 53.85%. Our results successfully achieved the induction and germination of adventitious shoots from the leaf callus, but the germination rate was still low and more studies should be done in the future.关键词
青钱柳/外植体/激素/愈伤组织/不定芽Key words
Cyclocarya paliurus/explant/hormone/callus/adventitious shoot分类
农业科技引用本文复制引用
阮氏钏,方升佐,尚旭岚,杨万霞..青钱柳愈伤组织不定芽诱导技术[J].南京林业大学学报（自然科学版）,2014,(2):52-56,5.基金项目
国家林业局林业科技成果推广项目（［2011］18号）；国家林业公益性行业科研专项项目（20090406）；江苏高校优势学科建设工程资助项目（ PAPD）；江苏高校协同创新计划 （）
