南京林业大学学报（自然科学版）Issue(2)：165-168,4.DOI:10.3969/j.issn.1000-2006.2014.02.032
大叶常绿杜鹃叶片离体培养及不定芽再生
In vitro leave culture of broad leaf evergreen rhododendron and its regeneration of adventitious buds
摘要
Abstract
Using the young leaves of aseptic broad leaf evergreen rhododendron seedling as the starting materials for this experiment, the culture conditions in vitro and regeneration of adventitious budswere studied. The results showed that:the best medium composition inducing adventitious buds directly from leaf explants were MS+ZT 1.0 mg/L+NAA 0.1 mg/L, which made the induction rate reach to 70%. The adventitious buds could also be induced from the callus indirectly, the best medium composition for which was MS+2,4-D 2.0 mg/L+KT 0.2 mg/L, the best multiplication medium for cal-lus was MS+ZT 0.5 mg/L+ IBA 0.1mg/L,the best medium for callus differentiation was MS+ZT 0.04 mg/L+NAA 0.01 mg/L, which made differentiation rate reach to 70% and the differentiation number of seedlings 6-10. The rate of rooting could reach to 90% by the medium of MS+NAA 0.2 mg/L +IBA 0.4 mg/L. The mixed compost contained four parts of humus soil, and one part of yellow sand, one part of perlite, which was quite suitable for evergreen rhododendron seed-lings transplanting in vitro, and the survival rate was 90%.关键词
大叶常绿杜鹃/离体培养/不定芽再生系统Key words
broad-leaf evergreen rhododendron/in vitro culture/regeneration system of adventitious buds分类
农业科技引用本文复制引用
吕秀立,魏翔莺,尹丽娟,黄芳,张春英..大叶常绿杜鹃叶片离体培养及不定芽再生[J].南京林业大学学报（自然科学版）,2014,(2):165-168,4.基金项目
国家自然科学基金项目 （）
