畜牧与饲料科学Issue(12)：27-30,4.
脱氢表雄酮对雄性小鼠睾丸组织发育的毒性作用
Toxic Effect of Dehydroepiandrosterone on the Testis Tissue Development of Male Mice
摘要
Abstract
32 Kunming male mice were randomly divided into 4 groups,which were fed with DHEA of 0 mg/kg (control group), 20 mg/kg(low-dose group), 40 mg/kg (medium-dose group) and 60 mg/kg (high-dose group) every day. The experiment lasted for 52 days. The mice in every group were killed on the 42 day-old and 72 day-old respectively. The testis was picked to make into paraffin sections and observe in microscope after HE staining. The results showed that the cross-section of seminiferous tubules deformed on 42 day-old and the number of spermatogenic cells and mature spermatozoa in each period all reduced in medium-dose group and high-dose group, compared with control group. In high-dose group, the hierarchy of spermatogenic cell in seminiferous tubule basically disappeared, bigger lacuna appeared and interstitium changed to different extent. On 72 day-old, the cross-section of seminiferous tubules deformed, basement membrane became thin accompanied by fracture and damaged extent of interstitium increased in medium-dose group and high-dose group. Abnormal sperms were found in medium dose group. In high-dose group, central blank area in seminiferous tubule was enlarged and mature sperm lacked. The results suggested that medium-dose and high-dose of DHEA had obvious toxic effects on the structure development of testicular tissue in growing mice.关键词
脱氢表雄酮/小鼠/睾丸/毒性作用Key words
dehydroepiandrosterone/mice/testis/toxic effect分类
生物科学引用本文复制引用
吕锦芳,姜锦鹏,宁康健,应如海,冯保明,檀永强..脱氢表雄酮对雄性小鼠睾丸组织发育的毒性作用[J].畜牧与饲料科学,2013,(12):27-30,4.基金项目
安徽省自然科学基金项目（11040606M88）；安徽科技学院兽医学重点学科基金项目（AKXK20101-2）。 （）
