畜牧与饲料科学Issue(1)：12-13,14,3.
甘露聚糖酶多克隆抗体的制备及其ELISA双抗夹心法的建立
Preparation of Polyclonal Antibodies of Mannanase and the Establishment of ELISA Double-antibody Sandwich Method
范文斌 1王和平 2刘永斌3
作者信息
- 1. 呼和浩特职业学院，内蒙古 呼和浩特 010051
- 2. 内蒙古农业大学，内蒙古 呼和浩特 010018
- 3. 内蒙古农牧业科学院，内蒙古 呼和浩特 010031
- 折叠
摘要
Abstract
The purpose of this study was to establish an effective method for detecting the expressed product by Escherichia coli transformed byβ-mannanase. Due to little expression amount and the changes of product structure, the conventional detection method couldn′t accurately judge the properties of expression product. So the selfmade antigen and anti-serum were used to establish ELISA. The detection results showed that E. coli transformed byβ-mannanase could successfully expressβ-mannanase.关键词
抗血清/β-甘露聚糖酶/酶联免疫法Key words
antiserum/β-mannanase/ELISA分类
农业科技引用本文复制引用
范文斌,王和平,刘永斌..甘露聚糖酶多克隆抗体的制备及其ELISA双抗夹心法的建立[J].畜牧与饲料科学,2014,(1):12-13,14,3.
