农业工程学报Issue(21)：19-25,7.DOI:10.3969/j.issn.1002-6819.2013.21.003
油菜抗裂角性鉴定方法的改进及试验
Optimization and experiment of assessment method for pod shatter resistance in Brassica napus L.
摘要
Abstract
Pod shattering is a serious problem for the production of rapeseed. Due to the lack of reliable and effective assessment methods, there has been little study on pod shattering. Therefore, it is very important to establish an accurate and readily available method to assess pod shattering for the development of pod shatter resistant lines. In order to establish a reliable assessment method, different methods for determining pod shatter resistance have been attempted. For example, a random impact test (RIT), rupture test, baking dry method, dropping seeds counting, filed observations, and so on. None of the methods, however, was proven efficient and easy for use at a wide range. Pod shattering occurs when dehydrated pods are stimulated by an exoteric force, and it is influenced by many factors. The key factor contributing to pod shattering is the moisture content of the pods and the force orientation. To make a random impact test more accurate and advantage, the effects of the moisture content of pods, impacting conditions on determination accuracy were analyzed. Measures such as using pods stored in an airproof bag overnight after being baked at 80℃ for 30 minutes to equilibrate moisture, recording cracked pods after every two minutes shaking rather than every one minute, as well as reducing the number of impact steel balls from 10 to 8 could increase the accuracy and efficiency of the assessment, and resulted in a more reliable distribution of the pod shatter resistance index (SRI) within a certain amount of materials. To verify the advantage of the modified parameters, forty-eight lines were assessed by RIT before or after modification. Results showed that the average value of pod SRI was 0.270, ranging from 0.032~0.767, and with a standard error of 0.0348 assessed using RIT before modification, whereas when assessed using RIT after modification, the figures were 0.272, 0~0.820, and 0.0248, respectively. The distribution of pod SRI in ranges of 0~0.1,>0.1~0.2,>0.2~0.3,>0.3~0.4,>0.4~0.5,>0.5~0.6,>0.6~0.7,>0.7~0.8, and>0.8 were 8, 11, 10, 9, 3, 2, 1, 2, and 0 assessed using RIT before modification, whereas assessed using RIT after modification, the figures were 10, 10, 9, 4, 6, 5, 1, 0, and 1, respectively. The pod SRI determined by the two procedures was significantly correlated with a correlation coefficient of 0.8844 (P=3.705×10-16). In conclusion, the modified version of the random impact test method possesses advantages to the same method before being improved in terms of repeatability, accuracy, and efficiency, so it can discriminate the levels of pod shatter resistance among exiting rapeseed varieties, and is very suitable for use in selection of pod shatter resistant lines in rapeseed breeding programs and quantification of pod shattering of lines involved in genetic and molecular studies.关键词
农作物/优化/水分/裂角性/随机碰撞法/油菜Key words
crops/optimization/water content/pod dehiscence/random impact test/rape分类
农业科技引用本文复制引用
彭鹏飞,李云昌,梅德圣,刘道敏,付丽,王会,桑世飞,陈玉峰,胡琼..油菜抗裂角性鉴定方法的改进及试验[J].农业工程学报,2013,(21):19-25,7.基金项目
国家科技支撑计划（2010BAD01B02；2011BAD35B04）；国家产业技术体系（CARS-13）；湖北省农业科技创新中心项目。 （）
