农业环境科学学报Issue(4):673-679,7.DOI:10.11654/jaes.2014.04.008
FTIR-NIR 研究镉胁迫对构树试管幼苗生长的影响与机理
FTIR-NIR Spectrum Study on Response and Mechanism of Broussonetia papyrifera Sprout Under Cadmium Stresses
摘要
Abstract
Broussonetia papyrifera, a popular tree in China, is tolerant to barren and drought soil conditions. It is also potential pollution-re-sistant. In this study, Fourrier Transform Infrared Spectrometry(FTIR)and Near Infrared Spectrum(NIR)were applied to study the dynam-ics of protein and other organic components in test-tube Broussonetia papyrifera sprout under different Cd stresses. Chlorophyll contents in Broussonetia papyrifera sprout leaves were also analyzed. Cadmium concentrations in sprout leaves increased, but chlorophyll contents de-creased with increasing Cd stresses. Under Cd stresses, The red edge position in fresh leaves of Broussonetia papyrifera sprout exhibited“blue-shift”, and the degree of the“blue-shift”showed a significant correlation with chlorophyll concentration. The red edge position of wavelength 710 nm in NIR can be used as an indicator of plant pollution by Cd. The intensities of absorption peaks 3010 cm-1, 1720 cm-1, 1636 cm-1, 1468 cm-1 and 892 cm-1 in FTIR increased with Cd stresses, implying that Broussonetia papyrifera sprout synthesized more pro-tein, polysaccharide, ester and other organic components under Cd stresses. FTIR-NIR is a useful technique to study the mechanisms of plant responses to heavy metal stress and is also a potentially quick method for monitoring plant heavy metal pollution.关键词
红外光谱/构树/镉胁迫/生理作用/叶绿素/化学组分Key words
infrared spectrum/Broussonetia papyrifera/Cd stress/physiological action/chlorophyll/chemical components分类
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王成,蒋泽平,李文青,邵文静,张婷,季峻峰..FTIR-NIR 研究镉胁迫对构树试管幼苗生长的影响与机理[J].农业环境科学学报,2014,(4):673-679,7.基金项目
国家“十一五”科技支撑项目“耐盐经济林木良种选育”(2006BAD09A04-01-06);南京市环保科技项目“典型工农业交错区土壤镉生物有效性影响因素及污染防治措施研究” ()