农业环境科学学报Issue(4)：680-686,7.DOI:10.11654/jaes.2014.04.009
Mn污染对木荷叶片抗氧化酶系统的影响
Effects of Manganese Contamination on Activities of Antioxidant Enzyme Systems in Leaves of Schima superba
摘要
Abstract
Manganese(Mn)is essential for plant growth, but excess Mn may impact antioxidant enzyme systems in plant. A soil culture ex-periment was performed to explore the effects of Mn contamination on various enzymatic antioxidants and non-enzymatic antioxidants in the leaves of Schima superba. Soil samples, collected from unmining section(WK), prospecting area(T), restored area(H), mining(K)and tailings(W)in a manganese mine of Hezhou, were used as Mn-polluted soils, and an unpolluted soil from Guilin, Guangxi was used as con-trol(CK). After 145-d of culture, leaves of S. superba were sampled and analyzed for the activities of superoxide dismutase(SOD), peroxi-dase(POD), catalase(CAT), and ascorbate peroxidase(APX)and the contents of ascorbic acid(AsA), glutathione(GSH),phytochelatins (PCs),total acid soluble SH,malondialdehyde(MDA), hydrogen peroxide(H2O2), and the produce rate of superoxide anion(O-2·). Concen-trations of Mn in roots, stems, and leaves increased with soil Mn concentrations. Compared to the control, plant biomass increased in WK and T samples, whereas decreased in the other soils, indicating stimulation of S. superba growth by Mn at low concentrations. The rate of O-2· production in the leaves was significantly higher in H than in control(P﹤0.05). The contents of H2O2 in leaves were increased by 65.7% and 96.3% for K and W, respectively. The leaf MDA was significantly(P﹤0.05)higher in mineral soils than in control, showing that the leaves were damaged by Mn pollution. The activities or contents of SOD, POD, AsA and GSH increased in order of CK﹤WK﹤T﹤H﹤K﹤W, in con-sistency with soil Mn concentrations. The POD enzyme activity in leaves in W soil was 2.62 times as much as that of control. Both CAT and APX enzymatic activities increased in WK and T, but decreased in the others, as compared with the control. The contents of GSH in leaves in K and W were about 1.66 and 1.79 times higher than those of the control.关键词
Mn污染/木荷/抗氧化酶系统Key words
manganese contamination/Schima superba/activity of antioxidant enzyme system分类
资源环境引用本文复制引用
苏银萍,刘华,于方明,李艺,陈孟林,周振明,李明顺..Mn污染对木荷叶片抗氧化酶系统的影响[J].农业环境科学学报,2014,(4):680-686,7.基金项目
国家自然科学基金项目（41161057）；广西科学研究与技术开发项目重大专项计划（桂科重1298002-6）；广西教育厅项目 （）
