热带亚热带植物学报Issue(1)：83-88,6.DOI:10.3969/j.issn.1005-3395.2014.01.013
巴西橡胶树两个蔗糖合成酶基因的克隆和表达分析
Cloning and Expression Analysis of Two Sucrose Synthase Genes from Hevea brasiliensis
摘要
Abstract
To investigate the function of sucrose synthase in Hevea brasiliensis growth and development, two genes encoding sucrose synthase, named as HbSS1 and HbSS2, were cloned from H. brasiliensis by RACE-PCR, and their expression patterns were analysed by semi-quantitative RT-PCR. The results showed that the whole length of HbSS1 cDNA was 2864 bp, encoding 806 amino acids;that of HbSS2 cDNA was 2815 bp, encoding 811 amino acids. Both HbSS1 and HbSS2 had typical characteristic of sucrose synthase in plants, containing a conserved phosphorylation site and two conserved domains. The expressions of HbSS1 and HbSS2 were detected in all tissues, their expressions were the highest in leaves and bark, respectively. It was suggested that HbSS1 and HbSS2 might be involved in growth and metabolism of various tissues, and they also had functional differentiation.关键词
巴西橡胶树/蔗糖合成酶/基因/表达Key words
Hevea brasiliensis/Sucrose synthase/Gene/Expression引用本文复制引用
朱家红,徐靖,畅文军,张治礼..巴西橡胶树两个蔗糖合成酶基因的克隆和表达分析[J].热带亚热带植物学报,2014,(1):83-88,6.基金项目
国家自然科学基金项目(31000313)；中央级公益性科研院所业务费专项资金项目(ITBB110201)；海南省重大科技项目子课题热带生物种质与基因资源研究(ZDZX2013023-1)资助 （31000313）
