热带亚热带植物学报Issue(2):147-154,8.DOI:10.3969/j.issn.1005-3395.2014.02.007
基于SRAP的叶子花种质资源遗传多样性及遗传关系分析
Genetic Diversity and Relationship of Bougainvillea Germplasm Resources Based on SRAP Markers
摘要
Abstract
In order to understand the genetic relationship of Bougainvillea germplasms, the SRAP (sequence-related amplified polymorphism) markers were firstly applied on analysis of genetic diversity and relationship among 48 Bougainvillea cultivars. The results showed that twenty-five primer pairs screened from 208 primer pairs ampliifed a total of 773 bands, of which 750 were polymorphic bands, and the percentage of polymorphic bands was 97.02%. The genetic similar coefficient of Bougainvillea germplasms ranged from 0.4058 to 0.8568, and UPGMA (Unweighted pair-group method arithmetic average) analysis showed that 48 cultivars could clustered into 4 groups with the genetic similarity coefifcient of 0.558. However, the cultivar B. glabra‘Formosa’ and B. spectabilis‘Spectabilis’ were clustered into a separate cluster, respectively, the other cultivars were clustered into two groups. The genetic relationships of all cultivars examined would be made clear based on SRAP molecular markers. The results could provide scientific foundation for using germplasm resource appropriately and improving the efifciency of screening new cultivars.关键词
叶子花/种质资源/品种/遗传多样性/SRAPKey words
Bougainvillea spectabilis/Germplasm resource/Cultivar/Genetic diversity/SRAP引用本文复制引用
唐源江,武晓燕,曹雯静..基于SRAP的叶子花种质资源遗传多样性及遗传关系分析[J].热带亚热带植物学报,2014,(2):147-154,8.基金项目
华侨大学高层次人才引进项目(2009BS506)资助 (2009BS506)