热带亚热带植物学报Issue(2)：172-178,7.DOI:10.3969/j.issn.1005-3395.2014.02.010
黄花梨bZIP的cDNA克隆与表达分析
Cloning and Expresssion Analysis of bZIP Gene from Pyrus pyrifolia‘Huanghua’
摘要
Abstract
In order to understand the function of PpbZIP of Pyrus pyrifolia‘Huanghua’, PpbZIP gene was cloned from lfower buds of‘Huanghua’ based on homologous gene of bZIP. The results showed that full length of cDNA of PpbZIP (GenBank accession No.:KC951877) was 1843 bp, containing an ORF of 1227 bp and encoding 408 amino acids. PpbZIP also shared 92%homology with bZIP of Malus × domestica. QRT-PCR analysis showed that the expression of PpbZIP gene increased at initiate dormancy stage and then decreased with dormancy releasing. It was suggested that PpbZIP could involve in the regulation of bud dormancy process of‘Huanghua’.关键词
黄花梨/bZIP/克隆/表达Key words
Pyrus pyrifolia‘Huanghua’/bZIP/Clone/Expression引用本文复制引用
宋燕春,李永裕,黄添毅,陈铁娇,吴少华..黄花梨bZIP的cDNA克隆与表达分析[J].热带亚热带植物学报,2014,(2):172-178,7.基金项目
高等学校博士学科点专项科研基金项目(20113515110011)；福建省科技计划重点项目(2012N0005)资助 （20113515110011）
