四川大学学报（自然科学版）Issue(6)：1355-1362,8.DOI:10.3969/j.issn.0490-6756.2013.06.033
麻疯树亚硝基谷胱甘肽还原酶（JcGSNOR）的克隆与功能分析
Molecular clone and functional analysis of S-nitrosoglutathione reductase/GSNOR from Jatropha curcas
摘要
Abstract
JcGSNOR was cloned by RT-PCR and RACE techniques from Jatropha curcas .The expres-sion situation of JcGSNOR in different organs of Jatropha curcas was detected by real time PCR .The result showed that ,JcGSNOR was expressed in all tissue detected .The expression level was the highest in the seed ,root and stem were more or less ,and leaf was the least .With bioinformatic analysis ,the JcGSNOR may be comprised of 379 amino acids ,to give the molecular weight of 40 .256 kDa and the pI value of 6 .74;Result analyzed by NCBI showed that ,JcGSNOR has up to 80% similarity with GSNOR of other plants ,which indicated JcGSNOR is a new member of GSNOR family .To identify the biochemi-cal activity of JcGSNOR ,the gene encoding JcGSNOR was cloned into saccharomyces cerevisiae .GSN-OR activity and growth curve of yeast were detected .This study indicated that ,JcGSNOR showed no obvious effect on grow th of yeast ,but enhanced the GSNOR activity significantly .关键词
麻疯树/亚硝基谷胱甘肽还原酶/基因克隆/真核表达Key words
Jatropha curcas/GSNOR/gene clone/eukaryotic expression分类
生物科学引用本文复制引用
马沅,陈放..麻疯树亚硝基谷胱甘肽还原酶（JcGSNOR）的克隆与功能分析[J].四川大学学报（自然科学版）,2013,(6):1355-1362,8.基金项目
国家自然科学基金 （）
