四川大学学报（自然科学版）Issue(6)：1391-1396,6.DOI:10.3969/j.issn.0490-6756.2013.06.039
13-脂氧合酶在拟南芥叶片衰老中的作用分析
Analysis of the role of 13-Lipoxygenases in Arabidopsis leaf senescence
摘要
Abstract
Promotion of leaf senescence is one of the most important function of the phytohormone Jas-monic acid (JA ) .In plants ,JA is initially synthesized by the reaction catalyzed by 13-lipoxygenase (LOX) .Four genes are predicted to encode 13-LOX in A rabidopsis genome ,however ,little is known a-bout their role in JA regulated leaf senescence .Expression pattern analysis by RT-PCR showed that LOX3 ,LOX4 and LOX6 genes expression were significantly enhanced in senescing leaves ,while the ex-pression of LOX2 genes decreased in late stage of leaf senescence dramatically .The 9-LOX activity defi-cient mutant lox1/lox5 was acquired and the 13-LOX activities in leaves at different developmental sta-ges were further measured using linolenic acid as substrate .The activity in senescing leaves was signifi-cantly higher than that in developing young leaves .The results indicated that LOX3 , LOX4 , LOX6 might play important roles in JA synthesis during leaf senescence and the importance of LOX2 might be impaired at the late stage of this process .关键词
衰老/脂氧合酶/茉莉酸/拟南芥Key words
senescence/lipoxygenase/JA/A rabidopsis分类
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孙炎锋,吕东,王丽,苗琛,林宏辉..13-脂氧合酶在拟南芥叶片衰老中的作用分析[J].四川大学学报（自然科学版）,2013,(6):1391-1396,6.基金项目
国家自然科学基金 （）
