西华大学学报（自然科学版）Issue(1)：39-42,78,5.DOI:10.3969/j.issn.1673-159X.2014.01.010
响应面分析法优化肠杆菌的发酵条件生产壳聚糖酶
Optimization of Culture Conditions Using the Method of Response Surface Methodology for Chitosanase Production by Enterobacter sp.YT21
摘要
Abstract
Optimization of culture conditions were conducted for chitosanase production by Enterobacter sp.YT21.Firstly Culture temperature , pH and inoculum volume were optimized by one-factor-at-a-time experiments , and then Box-Behnken design and response surface methodology were employed for further optimization of the chitosanase production .The results indicated that the optimal culture conditions were 31.74℃, pH 6.5 and 7.26%(V/V) inoculum, and the highest chitosanase activity obtained was 13.59 U/mL.Error of the actual value compared with the predicted one was only 0.43%, so Enterobacter sp.YT21 was proved to be a suitable potential microorganism for use in chitosanase production .关键词
肠杆菌/壳聚糖酶/发酵条件/响应面分析Key words
Enterobacter sp./chitosanase/culture condition/response surface methodology分类
生物科学引用本文复制引用
程仕伟,孙少华,张盈,陶春彦,朱琳,姜国正..响应面分析法优化肠杆菌的发酵条件生产壳聚糖酶[J].西华大学学报（自然科学版）,2014,(1):39-42,78,5.基金项目
山东省高等学校科技计划项目（J12LD02）；鲁东大学学生创新基金 （）
