西华大学学报（自然科学版）Issue(2)：88-92,5.DOI:10.3969/j.issn.1673-159X.2014.02.020
响应面法优化杏鲍菇下脚料多糖提取工艺
Optimization of Polysaccharide from the Residue of Pleurotus Eryngii Using Response Surface Methodology
摘要
Abstract
Total polysaccharides were extracted from the residue of Pleurotus eryngii by hot water extraction method .One-factor-at-a-time method and response surface methodology were used to select appropriate levels of liquid -material ratio, extraction time and temperature.A quadratic regression model describing the effects of three process conditions on the extraction rate of total polysaccha -rides was developed using a Box-Behnken experimental design .The results showed that the optimal extraction conditions were deter-mined as follows:liquid/material ratio of 26 mL/g, extraction temperature 87℃and extraction time 3.2 h.Under the optimized condi-tions, the extraction rate of polysaccharide from the residue of pleurotus eryngii reached 8.76%.关键词
杏鲍菇下脚料/多糖/热水浸提/响应面分析Key words
Pleurotus eryngii residue/polysaccharide/hot water extraction technology/response surface methodology分类
生物科学引用本文复制引用
张庆,林凯,袁春红,李可,肖奎,向文良..响应面法优化杏鲍菇下脚料多糖提取工艺[J].西华大学学报（自然科学版）,2014,(2):88-92,5.基金项目
四川省教育厅一般项目（13ZB0025）；西华大学重点科研基金项目（Z1220531）；榕珍菌业-西华大学产学研联合实验室 （）
