摘要
Abstract
Objective:To probe the effect of Gankyrin gene silence by RNAi on the proliferation and invasion of human cancer of coloncell.Methods:SiRNA ,designed in vivo and targeting Gankyrin were transfected into SW 620 cells by lipofectamine 2000.The proliferationand invasion ability of SW620 cells are investigated in affects of cell population doubling time , cell cycle, apoptotic and invasion by comparing with control group.Results:Present research showed that Gankyrin-siRNA group had apparently longer cell population doublingtime [(25.6 ±0.8)h vs(21.7 ±0.6)h, P <0.01], higher cell apoptotic rate[(8.93 ±0.21)℅ vs(1.31 ±0.06)℅, P <0.01] andlower cell invasion rate [(34.2 ±0.3)℅ vs (53.9 ±0.6)℅, P <0.01].Meantime, Gankyrin-siRNA group dramatically inhibited SW620 cell at G1 phase in comparison with the control group.Conclusion:Gankyrin-siRNA can effectively decrease the proliferation andinvasion of human colonic cancer cell ,which reveals that Gankyrin might be a potential target for gene therapy of colon cancer .关键词
结肠癌/Gankyrin/RNA干扰Key words
Colon cancer/Gankyrin/RNA interference分类
医药卫生
