山东中医杂志Issue(5)：388-390,3.
参芪益心方对CHF大鼠心肌细胞凋亡指数及Bax、Bcl-2表达的影响
Effect of Shenqi Yixin Formula on Myocardial Apoptosis Index, and Expressions of Bax and Bcl-2 in Chronic Heart Failure Rats
摘要
Abstract
Objective:To study the effect of Shenqi Yixin formula on myocardial apoptosis index,expression of Bax and Bcl-2 in chronic heart failure rats induced with doxorubicin. Methods:Ninety male rats were randomly divided into six groups: normal control group,model group,enalapril control group,the Qili Qiangxin capsule control group,the low dose Shenqi Yixin formula group,and the high dose Shenqi Yixin formula group. Chronic heart failure rat models were established using doxorubicin. Assay apoptosis rate via terminal deoxynucleotidyl transferase dUTP nick end labeling ( TUNEL ) essay was applied to test the myocardial apoptosis index,and expressions of Bax and Bcl-2 expression were tested with immunohistochemical methods. Results:Myocardial apoptosis indices of chronic heart failure rats in treatment groups were decreased with statistical difference compared with the model group ( P<0 . 05). The expression of Bax in each treatment group cardiomyocytes was decreased while the expression of Bcl -2 gene was increased,with statistical differences compared with the model group (P<0.05);the effect in high dose Shenqi Yixin formula group was the most obvious. Conclusion:Shenqi Yixin formula could modulate apoptotic genes to inhibit apoptosis, and therefore it could slow or reverse ventricular remodeling. Thus it could treat the chronic heart failure.关键词
参芪益心方/慢性心力衰竭/细胞凋亡Key words
Shenqi Yixin formula/chronic heart failure/apoptosis分类
医药卫生引用本文复制引用
金娟,吴丹,邹国良,刘莉..参芪益心方对CHF大鼠心肌细胞凋亡指数及Bax、Bcl-2表达的影响[J].山东中医杂志,2014,(5):388-390,3.基金项目
黑龙江省自然科学基金（编号QC2010091/C190402）；黑龙江省教育厅科学技术研究项目（编号12531615）。 （）
