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高灌蓝莓 CBF 基因的单核苷酸多态性分析

王甲威 林科 魏海蓉 刘庆忠

山东农业科学Issue(2):8-10,3.
山东农业科学Issue(2):8-10,3.

高灌蓝莓 CBF 基因的单核苷酸多态性分析

Single Nucleotide Polymorphism Analysis of CBF Gene in Highbush Blueberry

王甲威 1林科 2魏海蓉 1刘庆忠1

作者信息

  • 1. 山东省果树研究所/山东省果树生物技术育种重点实验室,山东 泰安 271000
  • 2. 泰山学院,山东 泰安 271021
  • 折叠

摘要

Abstract

With 21 highbush blueberry cultivars as materials , the VcCBF gene was cloned and its single nucleotide polymorphisms ( SNPs) were analyzed based on sequencing .Totally, 123 non repetitive sequences were cloned and 120 SNPs were identified with the SNPs occurrence rate as one SNP every 695 bp and the nu-cleotide diversity value (Pi) as 0.01251.In 120 SNPs, 62 of them were singleton mutant sites, 55 were double mutant sites and 3 were triple mutant sites .The analysis on base substitution ways showed 91 transi-tions and 26 transversions with the ratio of Ti/Tv as 3.5.Five haplotypes of VcCBF gene were identified from the 21 blueberry cultivars .

关键词

CBF/单核苷酸多态性( SNP)/蓝莓

Key words

CBF/Single nucleotide polymorphism/Blueberry

分类

生物科学

引用本文复制引用

王甲威,林科,魏海蓉,刘庆忠..高灌蓝莓 CBF 基因的单核苷酸多态性分析[J].山东农业科学,2014,(2):8-10,3.

基金项目

公益性行业(农业)科研专项 ()

山东农业科学

OACSTPCD

1001-4942

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