山东农业科学Issue(2)：13-16,4.
牛肠道病毒 RT -PCR 检测方法的建立及初步应用
Establishment and Application of RT-PCR for Detection of Bovine Enterovirus
摘要
Abstract
A reverse transcription PCR (RT-PCR) method for detection of bovine enterovirus (BEV) was established using a pair of specific primers designed based on the 3 D gene of BEV in GenBank .This method could specifically amplify a 732-bp fragment from BEV strain , but not from bovine viral diarrhea vi-rus (BVDV), infectious bovine rhinotracheitis virus (IBRV), bovine coronavirus (BCoV) and bovine rota-virus virus (BRV).The detection limit of this method was 10 -1 TCID50 of BEV.In a detection of 84 samples suspected with bovine disease , 21 samples were positive with the positive rate as 25%.关键词
牛肠道病毒/3D基因/RT-PCR/检测Key words
Bovine enterovirus/3D gene/RT-PCR/Detection分类
农业科技引用本文复制引用
侯佩莉,程洪兵,刘晓,王洪梅,何洪彬..牛肠道病毒 RT -PCR 检测方法的建立及初步应用[J].山东农业科学,2014,(2):13-16,4.基金项目
现代农业（奶牛）产业技术体系科学家岗位基金（Cars-37）；泰山学者海外特聘专家基金（tshw20100417）；国家自然科学基金（31072160）；济南留学人员创业计划（20120203）；山东省农业重大应用技术创新课题 （）
