山东农业科学Issue(3)：1-4,4.
脯氨酸代谢途径在调控水稻phyB突变体干旱胁迫耐性中的作用
Involvement of Proline Metabolic Pathway in Regulating Drought Tolerance in Rice phyB Mutant
摘要
Abstract
The expression levels of key genes involved in proline metabolic pathway were compared in rice wild type (WT)and phyB mutant.The results showed that dehydration treatment induced the expression of proline biosynthetic gene OsP5CS1 and OsOAT,but suppressed the expression of proline catabolic gene OsP5CDH.And the expression level of OsP5CS1 gene in phyB mutant was obviously higher than that in WT, which suggesting that phyB negatively regulated its expression.To explore the relation between high OsP5CS1 transcript level and stronger drought tolerance in phyB mutant,the OsP5CS1 transgenic tobacco was obtained. Water loss rate assays of detached leaves revealed that transgenic tobacco showed a slower water loss rate com-pared to non-transgenic tobacco.In addition,the leaf disk differentiation rate under high salinity of transgen-ic tobacco was higher than that of non -transgenic tobacco.These findings suggested that the regulation of phyB on proline metabolic pathway was one of the important factors contributing to stronger drought tolerance in phyB mutant.关键词
水稻/phyB突变体/脯氨酸/干旱/盐胁迫Key words
Rice/phyB mutant/Proline/Drought/Salt stress分类
生物科学引用本文复制引用
周晋军,刘蓬,尹静静,谢先芝..脯氨酸代谢途径在调控水稻phyB突变体干旱胁迫耐性中的作用[J].山东农业科学,2014,(3):1-4,4.基金项目
国家转基因生物新品种培育科技重大专项（编号2014ZX08001-005B）和国家自然科学基金青年科学基金项目（编号31300232）资助。 （）
