山东农业科学Issue(3)：54-57,4.
产纤维素酶低温菌株的分离鉴定
Isolation and Identification of Low-Temperature Strain Producing Cellulase
摘要
Abstract
In order to promote straw returning and speed up its degradation in the North greenhouse soil, using rotten straw as raw materials and corn straw powder as medium,16 strains were isolated with Congo Red plate method at 15℃.Then the enzyme activities of 16 strains were detected.L-13 had the highest enzyme activity of 1 679.61 U/ml.The mycelial morphology and colony characteristics of L-13 were observed,and a series of physiological and biochemical tests and 16S rDNA sequence analysis were conducted.The L -13 strain was preliminarily identified as Bacillus subtilis.Owing to growing fastly and high yielding of cellulase at 15℃,it could degrade the straw under low temperature rapidly.关键词
玉米秸秆/低温/纤维素酶Key words
Corn straw/Low temperature/Cellulase分类
生物科学引用本文复制引用
刘晓辉,高晓梅,桓明辉,邓春海,李杨,熬静,郭玲玲..产纤维素酶低温菌株的分离鉴定[J].山东农业科学,2014,(3):54-57,4.基金项目
国家农业科技成果转化资金项目 （）
