山东农业科学Issue(4)：31-35,5.
不同抗生素对刺槐遗传转化受体系统的影响
Effects of Different Antibiotics on Genetic Transformation Receptor System of Robinia pseudoacacia L.
摘要
Abstract
In this research , the effects of different antibiotics , kanamycin ( Kana ) , cefotaxime ( Cef ) , ampicillin(Amp), carbenicillin(Carb)and erythromycin(E)on receptor system of genetic transformation for Robinia pseudoacacia were studied with the cambium as explants .The results showed that Cef had the minimal toxicity to explants and the best antibacterial effect .The Cef at the concentration of 200 mg/L could complete-ly inhibite the proliferation of Agrobacterium tumefaciens.Different concentrations of Kana were used for resist-ance screening of Robinia pseudoacacia transgenic plants .The results indicated that the optimal concentration for callus differentiation was 100~125 mg/L, and the optimal one for rooting culture was 75 mg/L.关键词
刺槐/遗传转化/抗生素/形成层Key words
Robinia pseudoacacia/Genetic transformation/Antibiotics/Cambium分类
农业科技引用本文复制引用
韩义,孙超,秦柱南,王凯,曹帮华..不同抗生素对刺槐遗传转化受体系统的影响[J].山东农业科学,2014,(4):31-35,5.基金项目
山东省良种工程课题“农业生物资源创新利用研究”［鲁农良种字（2010）117号］；国家农业成果转化资金项目 （）
