世界中医药Issue(2)：141-144,4.DOI:10.3969/j.issn.1673-7202.2014.02.004
液相色谱-质谱法同时测定吴茱萸水提物中吴茱萸碱和吴茱萸次碱的含量
Simultaneous Determination of Evodiamine and Rutaecarpine in Water extracts of Evodia rutaecarpa by LC-MS
摘要
Abstract
Objective:To establish a LC-MS method for the simultaneous determination of evodiamine and tutaecarpine in water extracts of Evodia rutaecarpa Methods:A Welch Materials Xtimate-C18 (2.1 mm ×150 mm,3 μm) was used .The mobile phase consisting of a mixture of methanol and 10 mmol/L ammonium acetate (85:15) was delivered at a flow-rate of 0.2 mL/min.The column temperature was maintained at 30℃.The mass spectrometer was operated under the positive ion mode .Atomization pressure(N2) was 40 psi, volt-age was 4000v and temperature was 100℃.The drying gas ( N2) flow was 10 L/min, the drying gas temperature was 350℃.Results:The linear ranges were 2.02~504 ng/mL (r=0.9992) and 1.97~493.33 ng/mL (r=0.9999) for evodiamine and rutaecarpine .The method extraction recoveries of evodiamine and rutaecarpine were 87.8%~97.04%and 86.35%~98.22%respectively.The intra-and inter -day RSD were also lower than 10%.Conclusion:The method is simple , reliable and sensitive .It can be used for the phar-macokinetics research of water extracts of Evodia rutaecarpa .关键词
吴茱萸水提物/吴茱萸碱/吴茱萸次碱/液相色谱-质谱Key words
Water extracts of Evodia rutaecarpa/Evodiamine/Rutaecarpine/LC-MS分类
医药卫生引用本文复制引用
鄢良春,李波,华桦,廖利,赵军宁..液相色谱-质谱法同时测定吴茱萸水提物中吴茱萸碱和吴茱萸次碱的含量[J].世界中医药,2014,(2):141-144,4.基金项目
“重大新药创制”科技重大专项项目（编号2011ZX09401-304-（4-1））；国家重点基础研究发展计划（973计划）项目（编号2009CB522801）；国家自然科学基金面上项目 （）
