世界中医药Issue(2)：145-150,154,7.DOI:10.3969/j.issn.1673-7202.2014.02.005
吴茱萸及其主要成分的遗传毒性研究
Studies on the Genetic Toxicity of Evodia Rutaecarpa and its Main Components
摘要
Abstract
Objective:To investigate the genetic toxicity of evodia rutaecarpa and its main components , including evodiamine , rutae-carpin and limonin , in order to provide useful data for developing toxic Traditional Chinese Medicine .Methods:Ames test, CHL chro-mosome aberation assay and micronucleus assay of bone marrow cell in mice were used to test evodiamine , rutaecarpin , limonin and evo-dia rutaecarpa.Strains TA97,TA98,TA100,TA102 and TA1535 were used in the Ames test.There were five dose groups respectively for evodiamine, rutaecarpin and limonin as follows:0.0005, 0.005 mg/plate, 0.05 mg/plate, 0.5 mg/plate, 5 mg/plate.As a result, the results of the Ames assay were negative for various groups of evodiamine , rutaecarpin and limonin .In CHL chromosome aberration assay, the doses of evodiamine , rutaecarpin and limonin were 0.005 mg/mL, 0.05 mg/mL and 0.5 mg/mL respectively.Exposure time were 4 h and 24 h.Results:The result of the Evodiamine was negative .Aberration rate of 24 h exposure Rutaecarpin chromosome in-creased significantly compared with that of negative control group (P<0.05).Aberration rate of Limonin chromosome 0.05 and 0.5 mg/ml groups increased in 4 h and 24 h exposure compared with negative control group (P<0.05), the 0.005 mg/mL group was not significantly different compared with negative control group (P>0.05).In vivo micronucleus test with evodia rutaecarpa alcohol extract , there were 3 dosing groups:0.88, 3.52,10.55 g raw drug/kg, 4 days, once every day.The results was negative.Conclusion:no genet-ic toxicity was observed in evodia rutaecarpa alcohol extract .However , rutaecarpin and limonin shows some genotoxicity in vivo test , which requires further research to confirm .关键词
吴茱萸/遗传毒性/小鼠骨髓微核试验/Ames试验/染色体畸变试验Key words
Evodia rutaecarpa/Genotoxicity/Micronuclei assay/Ames test/Chromosome aberration assay分类
医药卫生引用本文复制引用
夏祺悦,刘燕萍,杨润芳,刘强强,卓衍蔷,李宏霞..吴茱萸及其主要成分的遗传毒性研究[J].世界中医药,2014,(2):145-150,154,7.基金项目
973项目有毒中药药效学和靶器官毒作用规律研究 （）
