世界中医药Issue(2)：151-154,4.DOI:10.3969/j.issn.1673-7202.2014.02.006
商陆皂苷甲致肾细胞毒性的研究
Study on Renal Cell Toxicity Induced by Esculentoside A
摘要
Abstract
Objective:To investigate the toxicity of Esculentoside A on HK-2 cells (Human renal tubular epithelial cells ).Methods:1) MTT assay was used to test the cell viability .2)The LDH in cells supernatant, SOD and MDA in the cells were detected .3)Morphologi-cal changes of the cells were observed through inverted phase contrast microscope and electron microscopy .4) Cell apoptosis was detec-ted by Hochest33342-PI dyeing and flow cytometry .Results:1) Esculentoside A could inhibit nephrocyte viability dependently on time and dose (IC50 was 149.11μg· mL-1).2)Esculentoside A could increase the activity of LDH in nephrocyte supernatant , reduce SOD activity and increase MDA content in nephrocytes .3 ) Esculentoside A could cause damage of nephrocyte structure , cell apoptosis and necrosis.Conclusion:It showed that large dose of Esculentoside A (more than 100 μg· mL-1) has toxicity on HK-2 cells and the mechanisms of toxicity may include cellular oxidative damage and cell apoptosis .关键词
商陆皂苷甲/HK-2细胞/肾细胞毒性Key words
Esculentoside A/HK-2 cells/Renal cell toxicity分类
医药卫生引用本文复制引用
周倩,姚广涛,金若敏,谢家骏..商陆皂苷甲致肾细胞毒性的研究[J].世界中医药,2014,(2):151-154,4.基金项目
国家重点基础研究发展计划（973计划）项目（编号2009CB522807）；国家“十二五”科技重大专项课题“中药安全评价技术平台” （）
