世界中医药Issue(3)：355-358,4.DOI:10.3969/j.issn.1673-7202.2014.03.031
丹红注射液调节大脑中动脉阻塞大鼠血清氨基酸代谢谱的实验研究
Research on Danhong Injection Adjusting Metabolic Spectrum of Serum Amino Acid in Cerebral Infarction Rats
郭志丽 1朱妍 1肖红斌 2王忠3
作者信息
- 1. 中国中医科学院，北京，100700
- 2. 陕西步长制药有限公司，西安，712000
- 3. 中国科学院大连化学物理研究所，大连，116023
- 折叠
摘要
Abstract
Objective:To determine the content of amino acid to clarify the curative effect and the functioning mechanism of DanHong injection on microscopic .Methods:OPA reversed phase high performance liquid chromatography ( HPLC ) , precolumn derivatization method fluorescence detection, gradient elution were used.Mobile phase A was 15mol/L Na2HP04-Na2B07 buffer (pH =7.95), B was acetonitrile -methanol -water (45:45:15, V/V/V).As for linear gradient elution, mobile phase B in 6 min increased from 5%to 17. 2%, increased from 17.2%to 52%in 6-35 min.Results:1) Fifteen kinds of amino acids were isolated within 35 min;2) using princi-pal component analysis , three groups were well separated;3) using the SPSS analysis of variance , there were six amino acids in the sep-aration between groups was statistically significant .Conclusion:Danhong injection can improve metabolic trend of amino acid in cerebral infarction rats serum through adjusting perturbed pathways.关键词
高效液相色谱/氨基酸代谢组学/脑梗死/丝氨酸代谢Key words
HPLC/Amino acid/Cerebral infarction/Serine metabolism/Metabolomics分类
医药卫生引用本文复制引用
郭志丽,朱妍,肖红斌,王忠..丹红注射液调节大脑中动脉阻塞大鼠血清氨基酸代谢谱的实验研究[J].世界中医药,2014,(3):355-358,4.
