色谱Issue(3)：230-234,5.DOI:10.3724/SP.J.1123.2013.11028
柱前衍生高效液相色谱法同时测定水性涂料中6种醛类化合物
Simultaneous determination of six aldehydes in water-based coatings by high performance liquid chromatography with pre-column derivatization
摘要
Abstract
A high performance liquid chromatography method with pre-column derivatization was developed for the simultaneous determination of formaldehyde,acetaldehyde,propionalde-hyde,benzaldehyde,n-valeraldehyde and p-tolualdehyde in water-based coatings. After ultra-sonic extraction with water, the samples were derivatized with 2,4-dinitrophenylhydrazine (2,4-DNPH)in acetonitrile under acidic condition,then filtrated by 0. 45 μm organic syringe filters for the determination. Systematic investigation was carried out on the dependence of the derivatization conditions such as the acidity regulator,pH value,reaction temperature,reaction time and other factors. The optimized conditions were as follows:the dilute hydrochloric acid solution as acidity regulator,the pH of the buffer solution of 3,the reaction temperature of 60℃ ,and the reaction time of 30 min. Under the conditions,a linear relation was achieved in the range of 0. 08-2. 0 mg / L for the peak area to concentration of the six aldehydes. The detection limits were 0. 05-2. 50 mg / kg. The recoveries of standard addition at the spiked levels of 2. 0, 4. 0,6. 0 mg / kg were 87. 0% - 112. 8% with the relative standard deviations( RSDs,n = 6)of 1. 12% -9. 54% . The results showed that this method has a wide linear range,good precision and accuracy,and it is suitable for the simultaneous determination of the six aldehydes in water-based coatings.关键词
柱前衍生/高效液相色谱法/醛类化合物/2,4-二硝基苯肼/水性涂料Key words
pre-column derivatization/high performance liquid chromatography ( HPLC )/aldehydes/2,4-dinitrophenylhydrazine/water-based coatings分类
化学化工引用本文复制引用
陈潜,周宇艳,程欲晓,林苗..柱前衍生高效液相色谱法同时测定水性涂料中6种醛类化合物[J].色谱,2014,(3):230-234,5.基金项目
国家质检总局“双打”质检公益专项基金项目（2012104020）． （）
