色谱Issue(3)：278-283,6.DOI:10.3724/SP.J.1123.2013.11010
细胞代谢组学用于羽扇豆醇干预人乳腺癌细胞 MCF-7的机理探究
Mechanism research on the lupeol treatment on MCF-7 breast cancer cells based on cell metabonomics
摘要
Abstract
The objective of this research is to investigate the suppressive effects of lupeol on MCF-7 breast cancer cells,and explore its mechanism on inhibiting the proliferation of MCF-7 cells based on cell metabonomics and cell cycle. Gas chromatography-mass spectrometry(GC-MS)was used in the cell metabonomics assay to identify metabolites of MCF-7 cells and MCF-7 cells treated with lupeol. Then,orthogonal partial least squares discriminant analysis(OPLS-DA)was used to process the metabolic data and model parameters of OPLS-DA were as fol-lows:R2 Ycum = 0. 988,Q 2 Ycum = 0. 964,which indicated that these two groups could be distin-guished clearly. The metabolites( VIP( variable importance in the projection)﹥ 1)were ana-lyzed by t-test,and finally,metabolites( t﹤0. 05)were identified to be biomarkers. Eleven metabolites such as butanedioic acid,phosphoric acid,L-leucine and isoleucine which had a significant contribution to classification were selected and preliminarily identified due to the accurate mass. Cell cycle assay was analyzed by FACSCalibur. Since the cells in the phase of G1 were increased significantly after the treatment of lupeol,we speculated that lupeol has a bloc-king effect on the generation of succinyl-CoA and the reaction of substrate phosphorylation of tricarboxylic acid cycle of MCF-7 cells. This study provided a novel approach to the mechanism research on the lupeol treatment on MCF-7 breast cancer cells based on cell metabonomics.关键词
气相色谱-质谱/羽扇豆醇/MCF-7 细胞/乳腺癌/抑制机理/正交偏最小方差判别分析/细胞代谢组学/细胞周期Key words
gas chromatography-mass spectrometry( GC-MS)/lupeol/MCF-7 cells/breast cancer/inhibition mechanism/orthogonal partial least squares discriminant analysis( OPLS-DA)/cell metabonomics/cell cycle分类
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史栋栋,况媛媛,王桂明,彭章晓,王彦,阎超..细胞代谢组学用于羽扇豆醇干预人乳腺癌细胞 MCF-7的机理探究[J].色谱,2014,(3):278-283,6.基金项目
国家自然科学基金项目（21175092，21105064）；国家重大科学仪器设备开发专项（2011YQ150072，2011YQ15007204，2011YQ15007207，2011YQ15007210）；上海市自然科学基金项目（12ZR1413600）． （）
