色谱Issue(3)：294-298,5.DOI:10.3724/SP.J.1123.2013.11041
多壁碳纳米管固相萃取净化-高效液相色谱法测定猪肉和鸡肉中的磺胺多残留
Analysis of sulfonamide residues in pork and chicken by high performance liquid chromatography coupled with solid-phase extraction using multiwalled carbon nanotubes as adsorbent
摘要
Abstract
A multi-residue analytical method based on solid-phase extraction(SPE)with multi-walled carbon nanotubes(MWCNTs)as sorbent was developed. The determination of the sul-fonamides(SAs)in pork and chicken was carried out by high performance liquid chromatogra-phy-ultraviolet detection( HPLC-UV). The clean-up conditions were optimized. The analytes were extracted by acetonitril and cleaned-up by MWCNTs SPE cartridge. The extract was re-dissolved with the Na 2 HPO 4 buffer( pH 5. 5- 6. 0)for loading,and was washed with acetone-hexane(5 :95,v / v),then eluted with acetone-dichloromethane(1 :1,v / v)from the column. The mobile phase used in the chromatographic separation consisted of a binary mixture of ace-tonitrile and 50 mmol / L NaH 2 PO 4 with the volume ratio of 7 :3. A wide linear range was 0. 01-1. 00 mg / L with the correlation coefficients above 0. 998. The limits of detection(S / N = 3)were 0. 003 mg / L,and the limits of quantification(S / N = 10)were 0. 01 mg / L. The average recover-ies were over 70% for the nine SAs in the spiked range of 0. 02-0. 2 mg / kg,with the relative standard deviations( RSDs)lower than 8% . This study indicated that the MWCNTs SPE car-tridge is efficient for the clean-up of the SAs in animal tissues or products,and the method is simple,accurate and suitable for the quantification of the SAs residues.关键词
固相萃取/高效液相色谱/磺胺/猪肉/鸡肉/多壁碳纳米管Key words
solid-phase extraction(SPE)/high performance liquid chromatography-ultraviolet detection( HPLC-UV)/sulfonamides( SAs)/pork/chicken/multi-walled carbon nanotubes (MWCNTs)分类
化学化工引用本文复制引用
赵海香,刘海萍,闫早婴..多壁碳纳米管固相萃取净化-高效液相色谱法测定猪肉和鸡肉中的磺胺多残留[J].色谱,2014,(3):294-298,5.基金项目
河北北方学院应用化学创新团队项目（ CXTD1306）；河北北方学院创新人才培育基金（ CXRC1302）． （）
