色谱Issue(3)：304-308,5.DOI:10.3724/SP.J.1123.2013.11023
离子排斥色谱法测定黄酒中的13种有机酸
Determination of organic acids in rice wine by ion-exclusion chromatography
摘要
Abstract
An ion-exclusion chromatographic method for the simultaneous determination of organic acids in rice wine was developed. An IC-Pak Ion Exclusion column(300 mm×7. 8 mm, 7 μm)was used at 50 ℃ . The mobile phases were H2SO4(phase A)and acetonitrile(phase B) (98 :2,v/v)at a flow rate of 0. 5 mL / min . The gradient elution program was as follows:0-40 min,0. 01mol/LH2SO4 to 0. 02 mol/LH2SO4;40 - 50 min,0. 01mol/LH2SO4 . The injection volume was 10 μL. The detection wavelength was set at 210 nm. The results showed that oxalic acid,maleic acid,citric acid,tartaric acid,malic acid,ascorbic acid,succinic acid,lactic, fumaric acid,acetic acid,propionic acid,isobutyric acid and butyric acid were completely sep-arated and determined in 30 min. The linear correlation coefficients were above 0. 9997 in the range of 0. 001-1. 000 g / L. Under the optimized conditions,the recoveries of organic acids in rice wine were in the range of 93. 4% -103. 8% with the relative standard deviations(RSDs,n =5)of 0. 1% - 1. 5% . This method is feasible,convenient,fast,accurate and applicable for the quantitative analysis of the organic acids in rice wine.关键词
离子排斥色谱法/有机酸/黄酒Key words
ion-exclusion chromatography(IEC)/organic acids/rice wine分类
化学化工引用本文复制引用
林晓婕,魏巍,何志刚,林晓姿..离子排斥色谱法测定黄酒中的13种有机酸[J].色谱,2014,(3):304-308,5.基金项目
福建省科技计划项目（2009R10033-3）；福建省财政专项福建省农业科学院科技创新团队建设基金项目（ STIF-Y05）． （）
