色谱Issue(4)：381-387,7.DOI:10.3724/SP.J.1123.2013.10038
一种混合模式反相弱阳离子交换色谱填料的制备及其保留机理
Preparation and retention mechanism of a mixed-mode reversed-phase/weak-cationic-exchange chromatographic packing
摘要
Abstract
A“mixed ligand”octyl-carboxylic co-bonded silica(OCS)packing was prepared by the method of“ click chemistry”. The resulting OCS packing was characterized by elemental analysis( EI)and Fourier transform infrared spectroscopy( FT-IR) to prove the successful immobilization of octyl and carboxylic groups on the surface of silica gel. Then the mixed-mode reversed-phase / weak-cationic-exchange( RP / WCX)retention mechanism of the OCS packing was quantitatively probed by studying the retention factors of a homologous series of three cat-ionic surfactants on the mixed-mode stationary phase column as a function of the ammonium concentration in the eluent or the number of methylene groups in the solute. The one-site and two-site mixed-mode retention models of the three cationic surfactants on the OCS phases were studied by investigating the logarithm and reciprocal relationships of retention factors and salt concentrations,demonstrating that the two-site model was more suitable for the description of the retention mechanism of the three cationic surfactants on the OCS phases. Furthermore,the individual RP or WCX contribution to total retention was obtained according to the mathemati-cal equations of two-site retention mechanism,which can provide some valuable guidance for the separation of real samples. This study developed the qualitative model of retention mecha-nism of the mixed-mode OCS packing,and a series of standard basic mixtures were well sepa-rated on the OCS packing,demonstrating the great application potential of OCS packing for the separation of various basic compounds.关键词
反相弱阳离子交换色谱固定相/混合模式色谱/保留机理/数学模型/色谱分离Key words
reversed-phase / weak-cationic-exchange ( RP / WCX ) chromatographic packing/mixed-mode chromatography( MMC)/retention mechanism/mathematic model/chromato-graphic separation分类
化学化工引用本文复制引用
彭西甜,冯钰锜..一种混合模式反相弱阳离子交换色谱填料的制备及其保留机理[J].色谱,2014,(4):381-387,7.基金项目
国家自然科学基金项目（91017013）；湖北省农业科学院青年基金（2013NKYJJ18）． （）
