水生生物学报Issue(4)：669-677,9.DOI:10.7541/2013.78
蛇鳄龟微卫星标记的开发及一个养殖群体的遗传多样性分析
ISOLATION OF MICROSATELLITE MARKERS IN THE COMMON SNAPPING TURTLE (CHELYDRA SERPENTINA L.) AND ITS APPLICATION IN GENETIC DIVERSITY ANALYSIS IN A CULTURED POPULATION
摘要
Abstract
To isolate and identify polymorphic microsatellite markers which would be used for evaluating the genetic diversity of Chelydra serpentina L. An enriched microsatellite library of C. serpentina was constructed by the mag-netic-bead enrichment method used biotinylated (CA)15 probe. Screened the enriched microsatellite library by PCR am-plification technique, seventy microsatellite sequences were obtained. To analyze genetic diversity of a cultured C. ser-pentina, forty eight pairs of primers were designed on the basis of the microsatellite sequences and 36 of that were screened by PCR amplification technique. In these microsatellite loci, the number of alleles per locus ranged from two to nine with an average of 4.361. The number of effective allele ranged from 1.461 to 7.767 with an average of 3.498. The size of allele fragment ranged between 56 and 342 bp. The observed heterozygosity varied from 0.067 to 1.000 with an average of 0.725. The expected heterozygosity varied from 0.316 to 0.850 with an average of 0.600. Polymorphism information content (PIC) ranged from 0.2655 to 0.8359 with an average of 0.5573. The average fixation index coeffi-cient of population was −0.214, which suggested surplus heterozygotes in the population. These results indicated that the cultured C. serpentina populations have relatively high genetic diversity.关键词
蛇鳄龟/微卫星标记/磁珠富集法/遗传多样性Key words
Chelydra serpentina/Microsatellite markers/Magnetic bead hybridization enrichment/Genetic diversity分类
生物科学引用本文复制引用
刘丽,刘海情,郭昱嵩,王中铎,刘楚吾..蛇鳄龟微卫星标记的开发及一个养殖群体的遗传多样性分析[J].水生生物学报,2013,(4):669-677,9.基金项目
广东省科技计划项目(2010B020308004)；广东省海洋渔业科技攻关与研发项目(A201008C03) （2010B020308004）
广东省海洋渔业科技攻关与研发项目(A201101B02)资助 （A201101B02）
