水生生物学报Issue(4)：678-683,6.DOI:10.7541/2013.79
凡纳滨对虾MT基因序列及其在卵巢发育和低温胁迫中的表达分析
SEQUENCE AND EXPRESSION ANALYSIS OF METALLOTHIONEIN FROM LITOPENAEUS VANNMEI
摘要
Abstract
A 425 bp Litopenaeus vannmei Metallothionein gene (LvMT) cDNA sequence was got from the SMART cDNA library of low temperature treated young Litopenaeus vannmei. The cDNA contained a 98 bp 5′ terminal un-translated region (UTR), a 150 bp 3′terminal UTR and a 177 bp open reading frame (ORF) sequence that encoded 58 amino acids (AA). The deduced amino acids sequence was rich in cysteine and Cys-X(1-3)-Cys structure which was classic structure in MT genes. Multiple alignment showed high identity of MT gene from different species, and the iden-tity of Litopenaeus vannmei and Homarus americanus MT was up to 72.4%. The realtime PCR analysis revealed that LvMT mRNA mainly expressed in ovary and high level expression was found in different ovary development stages. Furthermore, the expression of LvMT mRNA was up-regulated in the low temperature treated Litopenaeus vannmei hepatopancreas. The data is useful for the function analysis of LvMT in ovary development and low temperature stress.关键词
凡纳滨对虾/金属硫蛋白/低温/卵巢Key words
Litopenaeus vannmei/Metallothionein/Low temperature/Ovary分类
生物科学引用本文复制引用
彭金霞,陈晓汉,房振峰,韦嫔媛,胡睿宇,李咏梅,蒋伟明,陈秀荔,赵永贞,李文红..凡纳滨对虾MT基因序列及其在卵巢发育和低温胁迫中的表达分析[J].水生生物学报,2013,(4):678-683,6.基金项目
国家863计划课题(编号2012AA10A404) （编号2012AA10A404）
现代农业产业技术体系建设专项资金(编号:CARS-47)资助 （编号:CARS-47）
