水生生物学报Issue(5)：844-854,11.DOI:10.7541/2013.108
黄沙鳖白底板病病原菌的分离鉴定及6种毒力基因检测
CHARACTERIZATION OF WHITE PLASTRON DISEASE PATHOGENS AND DETECTION OF SIX KNOWN VIRULENCE GENES IN TRUOGX SINENSIS
摘要
Abstract
Pathogenetic bacteria were isolated from hearts and livers of the infected individual Truogx sinensis, cultured on conventional methods, and used as challenge to determine their infectiveness. Identities of the isolated pathogens were distinguished through API 20NE, their phylogenetic status were analyzed by 16S rRNA sequencing and their sen-sitivities to drugs were tested by K-B method. The six known virulence genes in the pathogens were detected by PCR. The results showed that, of the thirteen pathogens abtained, nine strains were Aeromonas hydrophila, and four strains were Aeromonas sobria. In phylogenetical relationship of the nine Aeromonas hydrophila, five strains were close to Aeromonas hydrophila ATCC 7966, and four strains were close to Aeromonas hydrophila QDC01, while four Aeromo-nas sobria were close to Aeromonas sobria ATCC 43979. Drug sesnsitive testes indicated that only thirteen strains were found to be highly sensitive to cefoperazone, and pathogens from different farms presented to be quite different in drug sensitivity. Virulence gene detection showed that Aer, Act and ahp was positive at 100%;hly and Alt, 92.31%;and Ahal, 76.92%. Four genotypes were found in pathogens isolated, being hly+Aer+Alt+Act+ahal+ahp+ and hly+Aer+Alt-Act+ahal+ahp+, mainly dominating in Aeromonas hydrophila and Aeromonas sobria, respectively, and strains with hly exhibited stronger pathogenicity.关键词
黄沙鳖/白底板病/嗜水气单胞菌/温和气单胞菌/毒力基因Key words
Truogx sinensis/White plastron disease/Aeromonas hydrophila/Aeromonas sobria/Virulence gene分类
农业科技引用本文复制引用
黄钧,曾桂忠,黄艳华,胡大胜,罗华平,施金谷,彭民毅,禤均成,覃丽芬,滕忠作..黄沙鳖白底板病病原菌的分离鉴定及6种毒力基因检测[J].水生生物学报,2013,(5):844-854,11.基金项目
广西自然科学基金项目(2011GXNSFA018065) （2011GXNSFA018065）
广西水产畜牧兽医局专项项目(桂渔牧财[2010]58号 （）
桂渔牧财[2011]52号)资助 （）
