水生生物学报Issue(5)：905-911,7.DOI:10.7541/2013.117
38株维氏气单胞菌分离株的AFLP基因分型研究
AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF AEROMONAS VERONII
摘要
Abstract
The AFLP genotyping about 38 Aeromonas veronii isolates and an Aeromonas veronii standard strain was conducted by synthetic universal joints and adapters which matched the specific primers. The results showed that the 39 strains of Aeromonas veronii augmentation were out 1080 visible strips by five selected primers. The lengths of the segments ranged from 50 to 1000 bp. Seven hundred and twenty seven of the total strips were polymorphism. Each primer combinations produced 154 bands in average. Average detection rate for polymorphism was 66.7%. By UPGMA method to strip the clustering analysis, a clustering branches tree, Aeromonas veronii was obviously divided into nine classes. The fourth group Ⅳ type contains genes 14 strains isolated, accounted for about 35.9% of the total bacterial count, distributed in five different areas. Presumably, it may be the major epidemic genotype of case disease to channel catfish. Aeromonas veronii had regional differences. And Aeromonas veronii in the same area showed genotype diver-sity.关键词
维氏气单胞菌/DNA扩增片段长度多态性(AFLP)/基因分型/指纹图谱/聚类分析Key words
Aeromonas veronii/Amplified fragment length polymorphism (AFLP)/Genetic diversity/Fingerprint/Clustering analysis分类
农业科技引用本文复制引用
汪开毓,牟巧凤,黄锦炉,陈德芳,黄凌远,王均,连海..38株维氏气单胞菌分离株的AFLP基因分型研究[J].水生生物学报,2013,(5):905-911,7.基金项目
教育部《长江学者和创新团队发展计划》创新团队项目(IRT0848)资助 （IRT0848）
