水生生物学报Issue(5):929-937,9.DOI:10.7541/2013.120
大麻哈鱼胚胎耳石微结构及其群体环境标记
STRUCTURE OF OTOLITHS DAILY GROWTH RING OF EMBRYO CHUM SALMON AND ENVIRONMENTAL MASS MARKING
摘要
Abstract
This study marked the eyed egg otoliths of chum salmon from Heilongjiang river and Suifenhe river, by regulating the environment temperature periodically and sustainedly. This experiment consisted of 4 groups:1 for con-trol, 2 and 3 for regulatling temperature groups and 4 for the group exposed to the open air for a stretch of time. 10000 chum salmon eyed eggs were used in the experiment. The otoliths of chum salmon formed as primordium at the begin-ning of eyed egg stage and the initial daily growth ring, made up of dark and bright calcium zone, which began to sur-round the core 5 days later and the daily increment width was (2.6±0.15)μm. Under the proper environmental condition, 30 daily growth rings would be formed during the embryo period, and we corroborated the daily growth ring in sagitta and lapillus. Otoliths marking experiment was conducted when the daily growth ring was stable. With the environmental water temperature changed periodically, the embryo otolith formed the marking area. The picture of daily growth ring, with the darker and brighter stripe and the different daily increment, which was marked by artificial regulating tem-perature, was different from the natural daily increment, and the rate of marking reached 100%. This experiment ob-tained precision marking pictures, established fishes otoliths marking and distinquishing technology, which was suited to artificial propagation marking in reproduction and stocking assess for salmons such as chum salmon et al. As the safe, effective, and cheap marking technology, fish otoliths marking method would be used in fish artificial reproduction and stocking widely.关键词
大麻哈鱼/耳石/日轮/环境标记Key words
Chum salmon/Otoliths/Daily growth ring/Environmental mass marking分类
生物科学引用本文复制引用
刘伟,战培荣,王继隆,唐富江..大麻哈鱼胚胎耳石微结构及其群体环境标记[J].水生生物学报,2013,(5):929-937,9.基金项目
农业部财政专项渔业种质资源保护项目(2010-2012) (2010-2012)
黑龙江省国际合作项目(WB06B16)资助 (WB06B16)