水生生物学报Issue(1)：58-67,10.DOI:10.7541/2014.08
温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响
COMBINED EFFECTS OF TEMPERATURE AND SALINITY ON THE PHYSIOLOGICAL OSMOTIC INDUCTION AND ANTIOXIDANT RESPONSES IN THE JUVENILE JAPANESE FLOUNDER (PARALICHTHYS OLIVACEUS)
摘要
Abstract
A factorial experiment was designed to evaluate the combined effects of temperature (20℃, 24℃ and 28℃) and salinity (10‰ and 30‰) on the osmotic pressure and antioxidant responses in the juvenile Japanese flounder (Paralichthys olivaceus). The results showed that the temperature and salinity caused no significant differences in the plasma cortisol of the juvenile Japanese flounder exposed in the experimental conditions for either 1 day (1 day after exposure, 1DAE) or 6 DAE. High temperature and low salinity (28℃, salinity10‰) significantly enhanced osmotic pressure level of the juvenileP. olivaceus in 1 DAE, but no significant difference in 6 DAE was observed. The Na+-K+-ATPase activity in the gill at 28℃ was significantly higher than that in 19℃ and 24℃ in 1 DAE, and the interactive effect between temperature and salinity on Na+-K+-ATPase activity was highly remarkable in 6 DAE. High temperature and low salinity increased SOD and CAT activity of juvenileP. olivaceus in 1 DAE, and the MDA content in the treatment (28℃, salinity 10‰) was significantly higher than the other treatments. The LPO content of juvenileP. olivaceus in salinity 10‰ was higher than that in the salinity 30‰ in 1 DAE at each temperature. There were no significant differences in liver SOD,CAT activity or MDA content in 1 DAE. Therefore,as euryhaline fish, P. olivaceus has very strong adaptability to environmental changes which allows them to tolerate conditions as high as 28℃ in temperature and as low as 10‰ in salinity. The changes of temperature and salinity may initially result in alterations of physiological activities and antioxidant responses, especially under high temperature and low salinity; and the physiological functions of flounders most likely recover to normal conditions after 6 days.关键词
温度/盐度/渗透压/皮质醇/Na+-K+-ATP酶/抗氧化酶/脂质过氧化/褐牙鲆幼鱼Key words
Temperature/Salinity/Osmotic pressure/Cortisol/Na+-K+-ATPase/Antioxidant enzyme/Lipid peroxida-tion/Juvenile Japanese flounder分类
农业科技引用本文复制引用
郭勤单,王有基,吕为群..温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J].水生生物学报,2014,(1):58-67,10.基金项目
上海市科学技术委员会项目(11PJ1404500) （11PJ1404500）
国家自然科学基金(31072228) （31072228）
高等学校博士学科点基金(20113104110002) （20113104110002）
上海市教委创新项目(10ZZ102) （10ZZ102）
上海高校水产学一流学科建设项目 （）
上海高校知识服务平台上海海洋大学水产动物遗传育种中心,(2F1206)资助 （2F1206）
